Matthew During


Matthew During

College of Medicine
Phone: 614-247-4351
Topics: Cancer research, Immunology


Molecular Virology, Immunology and Medical Genetics

Joint Appointments

Professor, Neurological Surgery
Professor, Neuroscience

Contact Information:


912 Biomedical Research Tower
460 W 12th Ave
Columbus, OH 43210
Phone: (614) 247-4351
Fax: (614) 688-0731


1978 B.S., University of Auckland, Human Biology
1982 M.D., University of Auckland, Medicine & Surgery
1987 Postdoctoral, Massachusetts Institute of Technology, Neuroscience/Biochemistry
2003 Sc.D., University of Auckland, Neuroscience & Genetics

Research Interests:

AAV vectors in the nervous system, Canavan disease, Parkinson's disease

Selected Publications:

During, M.J. and Spencer, D.D. 1993. Extracellular hippocampal glutamate and spontaneous seizure in the conscious human brain. Lancet. Vol. 341: 1607-1610.
During, M.J., Naegele, J., O'Malley, K.L. and Geller, A.I. 1994. Long-term behavioral recovery in Parkinsonian rats by an HSV vector expressing tyrosine hydroxylase. Science. Vol. 266: 1399-1403.
Kaplitt, M.G., Leone, P., Xiao, X., Samulski, R.J, Pfaff, D.W., O'Malley, K.L. and During, M.J. 1994. Long-term gene expression and phenotypic correction using adeno-associated virus vectors in the mammalian brain. Nature Genetics. Vol. 8: 148-154.
During, M.J., Leone, P., Davis, K.E., Kerr, D. and Sherwin, R.S. 1995. Glucose modulates rat substantia nigra GABA release in the freely-moving rat via an effect on ATP-sensitive potassium channels. J. Clin. Invest.. Vol. 95: 2403-2408.
During, M.J., Ryder K.M. and Spencer, D.D. 1995. Hippocampal GABA transporter function in temporal lobe epilepsy. Nature. Vol. 376: 174-177.
Geller, A.I., Naegele, J.R., O'Malley, K.L. and During, M.J. 1995. Behavioral effects and gene delivery in parkinson rat model. Science. Vol. 269: 856-857.
During , M.J. 1996. Gene therapy chronology. Science. Vol. 273: 416-417.
During, M.J. 1996. Gene therapy in New Zealand. Science. Vol. 272: 467.
During, M.J. 1996. Gene trial in New Zealand. Lancet. Vol. 348: 618.
During, M.J., Samulski, R.J., Leone, P., Kaplitt, M.G., Freese, A. Xiao, X., Elsworth, J.D., Roth, R.H., Sladek, J.R.Jr., O'Malley, K.L. and Redmond, D.E. 1998. In vivo expression of therapeutic human genes for dopamine production in the caudates of MPTP-treated monkeys using an AAV vector. Gene Therapy. Vol. 5: 820-827.
During, M.J., Xu, R., Young, D., Kaplitt, M.G., Sherwin, R.S. and Leone, P. 1998. Peroral gene therapy of lactose intolerance using an AAV vector. Nature Medicine. Vol. 4: 1131-1135.
Song, S., Wang, Y., Bak, S-Y, During, M.J. et al. 1998. Modulation of Rat Rotational Behavior by Direct Gene Transfer of Protein Kinase C into Nigrostriatal Neurons. J. Neurosci. Vol. 18: 4119-4132.
During, M.J., Janson, C.G. 1999. PNAs breach the blood-brain barrier. Nature Biotechnology. Vol. 17: 753-754.
Young, D., Lawlor, P., Leone, P., Dragunow, M., During, M.J. 1999. Environmental enrichment inhibits spontaneous neuronal apoptosis and prevents neuronal injury in the rat. Nature Medicine. Vol. 5: 448-453.
During, M.J., Symes, C.A., Lawlor, P.A., Lin, J., Dunning J., Fitzsimons, H.L., Poulsen, D., Leone, P., Xu, R., Dicker, B.L., Lipski, J., Young, D. 2000. An Oral Vaccine Against NMDAR1 with efficacy in Experimental Stroke and Epilepsy. Science. Vol. 287: 1453-1460.
Leone, P., Janson, C.G., Bilianuk, L., Wang, J., Sorgi, F. McPhee, S., Haung, L., Matolon, R., Kaul. R., Freese, A., Young, D., Xu, R., McPhee, S., Mee, E., During, M.J. 2000. Apartoacylase gene transfer to the mammalian nervous system with therapeutic implications for Canavan Disease. Ann. Neurol. Vol. 48: 27-38.
Leone, P; Janson, C, G; Bilaniuk, L; Wang, Z; Sorgi, F; Huang, L; Matalon, R; Kaul, R; Zeng, Z; Freese, A; McPhee, S, W; Mee, E; During, M, J; Bilianuk, L. 2000. Aspartoacylase gene transfer to the mammalian central nervous system with therapeutic implications for Canavan disease. Annals of neurology. Vol. 48, no. 1.
Telfeian, A, E; Federoff, H, J; Leone, P; During, M, J; Williamson, A. 2000. Overexpression of GluR6 in rat hippocampus produces seizures and spontaneous nonsynaptic bursting in vitro. Neurobiology of disease. Vol. 7, no. 4.
Dumon, K, R; Ishii, H; Fong, L, Y; Zanesi, N; Fidanza, V; Mancini, R; Vecchione, A; Baffa, R; Trapasso, F; During, M, J; Huebner, K; Croce, C M. 2001. FHIT gene therapy prevents tumor development in Fhit-deficient mice. Proceedings of the National Academy of Sciences of the United States of America. Vol. 98, no. 6.
Dumon, K, R; Ishii, H; Vecchione, A; Trapasso, F; Baldassarre, G; Chakrani, F; Druck, T; Rosato, E, F; Williams, N, N; Baffa, R; During, M, J; Huebner, K; Croce, C M. 2001. Fragile histidine triad expression delays tumor development and induces apoptosis in human pancreatic cancer. Cancer research. Vol. 61, no. 12.
During MJ, Kaplitt MG, Stern MB, Eidelberg D. 2001. Subthalamic GAD gene transfer in Parkinson disease patients who are candidates for deep brain stimulation. Human Gene Ther. Vol. 12: 1589-1591.
During, M, J; Kaplitt, M, G; Stern, M, B; Eidelberg, D. 2001. Subthalamic GAD gene transfer in Parkinson disease patients who are candidates for deep brain stimulation. Human gene therapy. Vol. 12, no. 12.
Fitzsimons, H, L; Mckenzie, J, M; During, M J. 2001. Insulators coupled to a minimal bidirectional tet cassette for tight regulation of rAAV-mediated gene transfer in the mammalian brain. Gene therapy. Vol. 8, no. 22.
Fitzsimons, H.L., McKenzie, J.M., During, M.J. 2001. Insulators coupled to a bidirectional tet cassette for tight regulation of rAAV-mediated gene transfer in the mammalian brain. Gene Therapy. Vol. 8: 1675-1681.
Janson, C, G; During, M J. 2001. Viral vectors as part of an integrated functional genomics program. Genomics. Vol. 78, no. 1-2.
Janson, C, G; McPhee, S, W; Leone, P; Freese, A; During, M J. 2001. Viral-based gene transfer to the mammalian CNS for functional genomic studies. Trends in neurosciences. Vol. 24, no. 12.
Janson, C, G; Ramesh, T, M; During, M, J; Leone, P; Heywood, J. 2001. Human intrathecal transplantation of peripheral blood stem cells in amyotrophic lateral sclerosis. Journal of hematotherapy & stem cell research. Vol. 10, no. 6.
Janson, C.G. During, M.J. 2001. Viral vectors as part of an integrated functional genomics program. Genomics. Vol. 78: 3-6.
Janson, C.G., McPhee, S.W.J., Leone, P., Freese, A., During, M.J. 2001. Viral-based gene transfer to the mammalian CNS for functional genomic studies. Trends in Neuroscience. Vol. 24: 705-712.
Mastakov, M, Y; Baer, K; Xu, R; Fitzsimons, H; During, M J. 2001. Combined injection of rAAV with mannitol enhances gene expression in the rat brain. Molecular therapy : the journal of the American Society of Gene Therapy. Vol. 3, no. 2.
Xu, R., Janson, C.G., Mastakov, M., Lawlor, P., Young, D., Mouravlev, A., Fitzsimons, H., Choi, K-L., Ma, H., Dragunow, M.,Chen, Q., Dicker, B., During, M.J. 2001. Quantitative comparison of expression with adeno-associated virus(AAV-2) brain-specific gene cassettes. Gene Therapy. Vol. 8: 1323-1332.
Xu, R; Janson, C, G; Mastakov, M; Lawlor, P; Young, D; Mouravlev, A; Fitzsimons, H; Choi, K, L; Ma, H; Dragunow, M; Leone, P; Chen, Q; Dicker, B; During, M J. 2001. Quantitative comparison of expression with adeno-associated virus (AAV-2) brain-specific gene cassettes. Gene therapy. Vol. 8, no. 17.
Cao, L., During, M.J., Xiao, W. 2002. Replication competent helper functions for recombinant AAV vector generation. Gene Therapy. Vol. 9: 1199-1206.
Fitzsimons, H.L., Bland, R., During, M.J. 2002. Promoters and regulatory elements that improve adeno-associated virus vector expression in the brain. Methods. Vol. 28: 227-236.
Luo, J., Kaplitt, M.G., Fitzsimons, H.L., Zuzga, D., Liu, Y., Oshinsky, M.L. and During, M.J. 2002. Subthalamic GAD gene therapy in a Parkinson's Disease rat model. Science. Vol. 298: 425-429.
Mastakov, M., Baer, K., During, M.J. 2002. Recombinant AAV-2 and AAV-5 mediated gene transfer in the mammalian brain: quantitative analysis of heparin co-infusion. Molecular Therapy. Vol. 5: 371-380.
Mastakov, M.Y., Baer, K., Symes, C.W., Leichtlein, C.B., Kotin, R.M., During, M.J. 2002. Immunological aspects of rAAV delivery to the mammalian brain. J. Virol. Vol. 76: 8446-8454.
Poulsen, D.J., Harrop, J.S., During, M.J. 2002. Gene therapy for spinal cord injury and disease. J. Spinal Cord Med. Vol. 25: 2-9.
Baer, K; Waldvogel, H, J; During, M, J; Snell, R, G; Faull, R L, M; Rees, M I. 2003. Association of gephyrin and glycine receptors in the human brainstem and spinal cord: an immunohistochemical analysis. Neuroscience. Vol. 122, no. 3.
Banks, W.A., During, M.J., Niehoff, M.L. 2003. Brain Uptake of the Glucagon-like Peptide-1 Antagonist Exendin(9-39) After Intranasal Administration. J. Pharmacol. Exp. Ther. Vol. 309: 469-475.
Boulis, N.M., Noordmans, A.J., Song, D.K., Imperiale, M.J., Rubin, A., Leone, P., During, M., Feldman, E.L. 2003. Adeno-associated viral vector gene expression in the adult rat spinal cord following remote vector delivery. Neurobiol. Dis.. Vol. 14: 535-541.
Dunning, J., During, M.J. 2003. Molecular mechanisms of learning and memory. Exp Rev Mol Med. Vol. 7: 1-11.
During, M.J., Cao, L., Zuzga, D.S., Francis, J.S., Fitzsimons, H.L., Jiao, X., Bland, R.J., Klugmann, M., Banks, W.A., Drucker, D.J., Haile, C.N. 2003. Glucagon-like peptide-1 receptor involvement in learning and neuroprotection. Nature Medicine. Vol. 9: 1173-1179.
During, M.J., Young, D., Baer, K., Lawlor, P., Klugmann, M. 2003. Development and optimization of adeno-associated virus vector transfer into the central nervous system. Methods Mol Med. Vol. 76: 221-236.
Klugmann, M., Symes, W.C., Klaussner, B.K., Leichtlein, C.B., Serikawa, T., Young, D., During, M.J. 2003. Identification and distribution of aspartoacylase in the postnatal rat brain. Neuroreport. Vol. 14: 1837-1840.
Lin, E.D., Richichi, C., Young, D., Baer, K., Vezzani, A., During, M.J. 2003. Recombinant AAV-mediated expression of galanin in rat hippocampus suppresses seizure development. Eur. J. Neurosci. Vol. 18: 2087-2092.
Waldvogel, H, J; Baer, K; Snell, R, G; During, M, J; Faull, R L, M; Rees, M I. 2003. Distribution of gephyrin in the human brain: an immunohistochemical analysis. Neuroscience. Vol. 116, no. 1.
Cannon, C.M., Abdallah, L., Tecott, L.H., During, M.J., Palmiter, R.D. 2004. Dysregulation of striatal dopamine signaling by amphetamine inhibits feeding in hungry mice. Neuron. Vol. 44: 509-520.
Cao L, Jiao X, Zuzga DS, Liu Y, Fong DM, Young D, During MJ. 2004. VEGF links hippocampal activity with neurogenesis, learning and memory. Nat Genet. Vol. 36: 827-835.
Francis, J.S., Dragunow, M., During. M.J. 2004. ATF-3 protects rat hippocampal neurons from in vivo injection of kainic acid. Mol Brain Res. Vol. 124: 199-203.
Harrop, J.S., Poulsen, D.J., Xiao, W., Freese, A., During, M.J. 2004. Gene transfer to spinal cord neurons and astrocytes by different AAV serotypes and promoters. Spine. Vol. 29: 2787-2792.
Kells, A.P., Fong, D.M., Dragunow, M., During, M.J., Young, D., Connor B. 2004. AAV-Mediated gene delivery of BDNF or GDNF is neuroprotective in a model of huntington disease. Mol Ther. Vol. 9: 682-688.
Lawlor, P.A., During, M.J. 2004. Gene Therapy for Parkinson's Disease. Exp Rev Mol Med. Vol. 2: 1-18.
Noordmans, A.J., Song, D.K., Noordmans, C.J., During, M.J., Fitzsimons, H.L., Imperiale, M.J., Boulis, N.M. 2004. Adeno-associated viral glutamate decarboxylase expression in the lateral nucleus of the hypothalamus reduces feeding behavior. Gene Therapy. Vol. 11: 797-804.
Pearson, A.G., Gray, C.W., Pearson, J.F., Greenwood, J.M., During, M.J., Dragunow, M. 2004. ATF3 enhances c-Jun-mediated neurite sprouting. Mol. Brain Res. Vol. 120: 38-43.
Richichi, C., Lin, E.D., Stefanin, E., Collela D., Grignaschi, G., Sperk, G., During, M.J., Vezzani, A. 2004. Anticonvulsant and antiepileptic effects mediated by adeno-associated virus vector NPY expression in the rat hippocampus. Eur. J. Neurosci. Vol. 24: 3051-3059.
Szumlinski KK, Dehoff MH, Kang SH, Frys KA, Lominac KD, Klugmann M, Rohrer J, Griffin W 3rd, Toda S, Champtiaux NP, Berry T, Tu JC, Shealy SE, During MJ, Middaugh LD, Worley PF, Kalivas PW. 2004. Homer proteins regulate sensitivity to cocaine. Neuron. Vol. 43: 401-413.
Young, D., During, M.J. 2004. Using the immune system to target epilepsy. Adv Exp Med Biol. Vol. 548: 134-144.
Baldock PA, Sainsbury A, Allison S, Lin EJ, Couzens M, Boey D, Enriquez R, During M, Herzog H, Gardiner EM. 2005. Hypothalamic Control of Bone Formation: Distinct Actions of Leptin and Y2 Receptor Pathways. J Bone Miner Res. Vol. 20: 1851-1857.
Klugmann, M., Leichtlein, C., Symes, W.C., Klaussner, B.C., Brooks, A.I., Young, D. During, M.J. 2005. A novel role of circadian transcription factor DBP in hippocampal plasticity. Mol. Cell. Neurosci.
Klugmann, M., Leichtlein, C.B., Symes, C.W., Serikawa, T., Young, D. During, M.J. 2005. Restoration of aspartoacylase activity in CNS neurons does not ameliorate motor deficits and demyelination in a model of Canavan disease. Mol. Ther. Vol. 11: 745-753.
Klugmann, M., Symes, W.C., Leichtlein, C., Klaussner, B.C., Dunning, J., Fong, D., Young, D., During, M.J. 2005. AAV-mediated overexpression of short and long Homer 1 proteins differentially affect memory and epileptogenesis in adult rats. Mol. Cell. Neurosci. Vol. 28: 347-360.
Lominac KD, Szumlinski KK, Oleson EB, Klugman M, Schwarz MK, Seeburg PH, During MJ, Worley PF, Kalivas PW. 2005. Distinct roles for different Homer1 isoforms in behaviors and associated prefrontal cortex function. J. Neurosci. Vol. 25: 11586-11594.
Rashidian J, Iyirhiaro G, Aleyasin H, Rios M, Vincent I, Callaghan S, Bland RJ, Slack RS, During MJ, Park DS. 2005. Multiple cyclin-dependent kinases signals are critical mediators of ischemia/hypoxic neuronal death in vitro and in vivo. Proc Natl Acad Sci USA. Vol. 102: 14080-14085.
Szumlinski KK, Lominac KD, Oleson EB, Walker JK, Mason A, Dehoff MH, Klugman M, Cagle S, Welt K, During M, Worley PF, Middaugh LD, Kalivas PW. 2005. Homer isoforms differentially regulate Cocaine-induced neuroplasticity. Neuropschopharmacol.
Szumlinski KK, Lominac KD, Oleson EB, Walker JK, Mason A, Dehoff MH, Klugman M, Cagle S, Welt K, During M, Worley PF, Middaugh LD, Kalivas PW. 2005. Homer2 is necessary for EtOH-induced neuroplasticity. J Neurosci. Vol. 25: 7054-7061.
Xu, R., Rahimi, M., Ma, H., Fung, P., Chang, C., Xu, S., During, M.J. 2005. Stability of infectious adeno-associated viral vector in gene delivery. Med. Sci. Monit. Vol. 11: BR305-BR308.
During, MJ, Cao, L. 2006. VEGF, a mediator of the effect of experience on hippocampal neurogenesis. Curr. Alzheimer Res. Vol. 3: 1017-1020.
Mouravlev, A., Dunning, J., Young, D., During, M.J. 2006. Somatic gene transfer of cyclic AMP response element-binding protein attenuates memory impairment in aging rats. Proc. Natl. Acad. Sci., USA. Vol. 103: 4705-4710.
Olson, V., Heusner, C.I., Bland, R.I., During, M.J., Weinshenker, D., Palmiter, R.D. 2006. Role of noradrenergic signaling by the nucleus tractus solitarious in mediating opiate reward. Science. Vol. 311: 1017-1020.
Robinson, S., Sotak, B.N., During, M.J., Palmiter, R.D. 2006. Local dopamine production in the dorsal striatum restores goal-directed behavior in dopamine-deficient mice. Behav. Neurosci. Vol. 120: 196-200.
Tappe, A., Klugmann, M, Luo, C., Hirlinger, D., Agarwal, N., Benrath, J., Ehrengruber, M., During, MJ, Kuner, R. 2006. Synaptic scaffolding protein Homer 1a protects against chronic inflammatory pain. Nat. Med.
Aleyasin H; Rousseaux MW; Phillips M; Kim RH; Bland RJ; Callaghan S; Slack RS; During MJ; Mak TW; Park DS. 2007. The Parkinson's disease gene DJ-1 is also a key regulator of stroke-induced damage. Proceedings Of The National Academy Of Sciences Of The United States Of America. Vol. 104, no. 47. (November 20): 18748.
Feigin A; Kaplitt MG; Tang C; Lin T; Mattis P; Dhawan V; During MJ; Eidelberg D. 2007. Modulation of metabolic brain networks after subthalamic gene therapy for Parkinson's disease. Proceedings Of The National Academy Of Sciences Of The United States Of America. Vol. 104, no. 49. (December 4): 19559.
Fitzgibbon, B, M; Fairhall, S, L; Kirk, I, J; Kalev-Zylinska, M; Pui, K; Dalbeth, N; Keelan, S; Robinson, E; During, M; McQueen, F M. 2008. Functional MRI in NPSLE patients reveals increased parietal and frontal brain activation during a working memory task compared with controls. Rheumatology (Oxford, England). Vol. 47, no. 1.
Sørensen AT; Kanter-Schlifke I; Carli M; Balducci C; Noe F; During MJ; Vezzani A; Kokaia M. 2008. NPY gene transfer in the hippocampus attenuates synaptic plasticity and learning. Hippocampus. Vol. 18, no. 6. (January 1): 564.
Chen X; Rzhetskaya M; Kareva T; Bland R; During MJ; Tank AW; Kholodilov N; Burke RE. 2008. Antiapoptotic and trophic effects of dominant-negative forms of dual leucine zipper kinase in dopamine neurons of the substantia nigra in vivo. The Journal Of Neuroscience: The Official Journal Of The Society For Neuroscience. Vol. 28, no. 3. (January 16): 672.
Adesnik H; Li G; During MJ; Pleasure SJ; Nicoll RA. 2008. NMDA receptors inhibit synapse unsilencing during brain development. Proceedings Of The National Academy Of Sciences Of The United States Of America. Vol. 105, no. 14. (April 8): 5597.
Franich NR; Fitzsimons HL; Fong DM; Klugmann M; During MJ; Young D. 2008. AAV vector-mediated RNAi of mutant huntingtin expression is neuroprotective in a novel genetic rat model of Huntington's disease. Molecular Therapy: The Journal Of The American Society Of Gene Therapy. Vol. 16, no. 5. (May 1): 947.
Jung AE; Fitzsimons HL; Bland RJ; During MJ; Young D. 2008. HSP70 and constitutively active HSF1 mediate protection against CDCrel-1-mediated toxicity. Molecular Therapy: The Journal Of The American Society Of Gene Therapy. Vol. 16, no. 6. (June 1): 1048.
Noè F; Pool AH; Nissinen J; Gobbi M; Bland R; Rizzi M; Balducci C; Ferraguti F; Sperk G; During MJ; Pitkänen A; Vezzani A. 2008. Neuropeptide Y gene therapy decreases chronic spontaneous seizures in a rat model of temporal lobe epilepsy. Brain: A Journal Of Neurology. Vol. 131, no. Pt 6. (June 1): 1506.
Sørensen AT; Kanter-Schlifke I; Lin EJ; During MJ; Kokaia M. 2008. Activity-dependent volume transmission by transgene NPY attenuates glutamate release and LTP in the subiculum. Molecular And Cellular Neurosciences. Vol. 39, no. 2. (October 1): 229.
Riban V; Fitzsimons HL; During MJ. 2009. Gene therapy in epilepsy. Epilepsia. Vol. 50, no. 1. (January 1): 24.
Sørensen AT; Nikitidou L; Ledri M; Lin EJ; During MJ; Kanter-Schlifke I; Kokaia M. 2009. Hippocampal NPY gene transfer attenuates seizures without affecting epilepsy-induced impairment of LTP. Experimental Neurology. Vol. 215, no. 2. (February 1): 328.

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